This page is a list of working methods to uncheck a box in a PDF, depending on the type of PDF and tools available.
TL;DR:
This is a quick option for simple interactive PDFs without needing additional software.
It requires any modern web browser like Chrome, Edge, or Firefox.
Here are the simple steps:

Google Chrome is a web browser that also offers PDF reader features. It allows you to interact with fillable PDF forms, filling fillable PDF forms, for instance.
For PDF forms with checkboxes, it allows you to click to check/uncheck it.
If you find the checkbox isn’t responsive, that means the PDF is not interactive/flattened. In that case, a PDF markup tool is needed to uncheck the checkbos.
To uncheck a non-interactive PDF, a simple way is to use a free online PDF editor to white out unselectable boxes in a PDF.

PDFgear is free to use, there’s no signup, and there’s no watermark.
PDFgear’s online editor uses a rectangle shape to cover the unwanted checkmark. However, you should note that this can be recovered unless you printed the file.
Use the Print file feature instead of the Download file feature to make sure the markup history is gone.
This method works well for fillable PDF forms and allows direct editing of check boxes.

Uncheck in Acrobat
Adobe Acrobat’s Prepare Form tool is designed to detect potential form elements, including non-interactive checkboxes, and convert them into real, interactive form fields.
If your checkboxes are part of a scanned document, try running OCR (Text Recognition) first using Scan & OCR > Recognize Text, then run Prepare Form — this improves detection dramatically.
If you can’t uncheck boxes in a PDF, not because the PDF is scanned, but because the PDF is protected, then you’ll need to unlock the Permissions Password (Edit restrictions).
Viable methods are as follows:
Using Adobe Acrobat Pro:
Print the document to PDF using Microsoft Print to PDF (Windows) or Save as PDF (macOS).
This flattens the document, removing interactive checkboxes but allowing you to:
Limitation: You lose interactivity (can’t toggle form fields anymore).
Some PDF tools can ignore or override basic permission restrictions (but not open-password or encryption).
Examples: PDFgear
If you find Acrobat fails to detect certain checkboxes in a scanned PDF, that usually results from:
If you try to uncheck a box in PDF but it won’t uncheck whatsoever, it may be a flat image or part of the PDF background. In that case, use tools like PDFgear or Adobe Acrobat to add a white rectangle over the checked box to “cover” it. Learn how to white out on PDF for free.
It may be locked due to document restrictions or security settings. You’ll need permission to edit, or use software that can remove restrictions (e.g., Adobe Acrobat Pro).